Composite

Part:BBa_K5311016:Design

Designed by: Carlos Perez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-27)


MG_010 + RNA thermometer ForU + sfGFP + His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For this composite part we have codon optimized the CDS for the RNA Thermometer to be more compatible with C. acnes, we also introduced the standard prefix and sufixes in our sequence to ensure a proper alignment for the Gibson Assembly.


Source

This part was created by using the corresponding sequences on this registry: MG10: Part:BBa_J23119; RNA Thermometer: Part:BBa_K115002; sfGFP: Part:BBa_K5311007 and the His-tag corresponds to a sequence of 6 Hystidines

References